p53-gfp fusion protein-expressing construct (SpectraGenetics Inc)
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P53 Gfp Fusion Protein Expressing Construct, supplied by SpectraGenetics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p53-gfp+fusion+protein-expressing+construct/p53+gfp+fusion+protein+expressing+construct/pmc03488463-308-25-7
Average 90 stars, based on 1 article reviews
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1) Product Images from "An arrayed human genomic library constructed in the PAC shuttle vector pJCPAC-Mam2 for genome-wide association studies and gene therapy"
Article Title: An arrayed human genomic library constructed in the PAC shuttle vector pJCPAC-Mam2 for genome-wide association studies and gene therapy
Journal: Gene
doi: 10.1016/j.gene.2012.01.011
Figure Legend Snippet: Live-cell imaging of p53-GFP-expressing Saos-2 cells. Saos-2 cells were transfected as described previously with pJCPAC-Mam1-p53-GFP. Three days post-transfection, Saos-2 cells were photographed and UV and white light images were merged. (A). Exogenous p53-GFP localizes predominantly to the nuclei of Saos-2 cells. (B). Membrane blebbing, a hallmark event in apoptosis occurs in a subset of p53-GFP-expressing Saos-2 cells.
Techniques Used: Live Cell Imaging, Expressing, Transfection
Related Articles
Transfection:Article Title: An arrayed human genomic library constructed in the PAC shuttle vector pJCPAC-Mam2 for genome-wide association studies and gene therapy Article Snippet: A p53-GFP cassette (Gift of Jon Jarvik, SpectraGenetics) was subcloned into the unique NotI site of pJCPAC-Mam1 and Saos-2 cells were also transfected with this p53-GFP fusion protein-expressing construct using PEI as previously described. Construct:Article Title: An arrayed human genomic library constructed in the PAC shuttle vector pJCPAC-Mam2 for genome-wide association studies and gene therapy Article Snippet: A p53-GFP cassette (Gift of Jon Jarvik, SpectraGenetics) was subcloned into the unique NotI site of pJCPAC-Mam1 and Saos-2 cells were also transfected with this p53-GFP fusion protein-expressing construct using PEI as previously described. Live Cell Imaging:Article Title: An arrayed human genomic library constructed in the PAC shuttle vector pJCPAC-Mam2 for genome-wide association studies and gene therapy Article Snippet: A p53-GFP cassette (Gift of Jon Jarvik, SpectraGenetics) was subcloned into the unique NotI site of pJCPAC-Mam1 and Saos-2 cells were also transfected with this p53-GFP fusion protein-expressing construct using PEI as previously described. Expressing:Article Title: An arrayed human genomic library constructed in the PAC shuttle vector pJCPAC-Mam2 for genome-wide association studies and gene therapy Article Snippet: A p53-GFP cassette (Gift of Jon Jarvik, SpectraGenetics) was subcloned into the unique NotI site of pJCPAC-Mam1 and Saos-2 cells were also transfected with this p53-GFP fusion protein-expressing construct using PEI as previously described. |